Composite

Part:BBa_K4649010:Design

Designed by: Sophia Eowyn Hsu   Group: iGEM23_EastCoastBioCrew   (2023-09-11)


PqqC + gcd codon optimized for Rhizobium tropici


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2100
    Illegal XbaI site found at 3328
    Illegal SpeI site found at 3322
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2100
    Illegal SpeI site found at 3322
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2100
    Illegal BamHI site found at 2049
    Illegal BamHI site found at 3316
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2100
    Illegal XbaI site found at 3328
    Illegal SpeI site found at 3322
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2100
    Illegal XbaI site found at 3328
    Illegal SpeI site found at 3322
    Illegal NgoMIV site found at 479
    Illegal NgoMIV site found at 1033
    Illegal NgoMIV site found at 1309
    Illegal NgoMIV site found at 1362
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 2895
    Illegal NgoMIV site found at 3052
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for R. tropici


Source

Escherichia coli strain K12 genome, Agrobacterium radiobacter strain K84 genome


References